The microscope makers

A small community of scientists has taken a do-it-yourself approach to microscopy: when the right tool for the job doesn’t exist, make it.

While pursuing a bioengineering PhD at the University of Pennsylvania in Philadelphia, Wesley Legant ran into a frustrating roadblock: he had ideas, but the equipment to carry them out didn’t yet exist.

With an interest in cell mechanics and motility, Legant was developing tools to measure the forces that cells exert on their environment. He embedded fluorescent beads in the material surrounding a growing mammalian cell so that as the cell moved, it would deform the material, moving the beads. By measuring how much the beads moved, Legant could calculate the forces exerted by the cell. Still, he had difficulty getting accurate data. “The tools were successful, but I was quickly coming up against limitations in available microscopes,” he says. Read more in Nature.